WP4

Objectives:

1. Design and construct tissue microarrays (TMAs) with appropriate control tissues, including the tumour tissues (PCa, BCa, NB), metastasis target tissues (bone, liver, brain), as well as tissues with high immune cell content (lymph node, appendix)
2. Collect the clinical and pathological patient data associated with the tissue samples into a clinical database available for the project partners
3. Design and optimize antibody panels for multiplex fluorescence immunohistochemistry (mfIHC)
4. Perform mfIHC on TMAs and analyse scanned images for single-cell resolution phenotyping

Expected Results: This project will identify the main immune cell subsets and their activation/inhibition states within established metastases of PCa, BCa, and NB. The collected single-cell spatial imaging data will uncover cellular interactions and proximities of immune cells with each other and with tumour cells in clinical patient samples. The imaging data will be indexed and integrated with collected clinical data and other data in collaboration with PhD12 and PhD13.